ТИПИРОВАНИЕ ТКАНЕЙ МЕТОДАМИ ПЦР И КАПИЛЛЯРНОГО ЭЛЕКТРОФОРЕЗА: РЕАЛЬНОСТЬ, ВОПРОСЫ И ПЕРСПЕКТИВЫ

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Целью текущего обзора является раскрытие новых перспектив для РНК- и ДНК-анализа в рутинной практике судебной биологии. В обзоре рассмотрены современные тканевые маркеры, основанные на матричных РНК (мРНК), методы их тестирования и подходы для совмещения РНК-анализа со стандартными протоколами исследования судебного материала. Исходя из практического опыта зарубежных исследователей, новые маркеры вполне совместимы с классическими протоколами, включая использование методов ПЦР и капиллярного электрофореза, и могут быть применены после постановки иммунологических тестов, что усиливает чувствительность и не требует принципиального отказа от их использования. На сегодняшний день сформированы относительно готовые мультиплексные тканевые наборы, подготовленные для коммерческих реализаций в скором будущем. Популяционное генетическое разнообразие в экспрессии генов определяет возможность разработки отечественных панелей мРНК-маркеров на базе существующих зарубежных панелей.

Об авторах

А. С. Бавыкин

ООО «Ниармедик плюс»

Автор, ответственный за переписку.
Email: andrey.bavykin@nearmedic.ru
к.м.н., LLC «Nearmedic Plus› 125252, г. Москва Россия

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